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KMID : 0366119730010020105
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Korean Journal of Applied Microbiology & Bioengineering
1973 Volume.1 No. 2 p.105 ~ p.113
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Studies on the Effect of Biotin Vitamers as a Growth Factors in the L-Glutamic Acid Fermentation
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Abstract
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The effect of biotin and biotin vitamer on the fermentative production of L-glutamic acid. (L-GA) by Brevibacterium flavum was studied. And results were as follows.
1) L-GA production in the medium containing 10% Glucose was the best at the concentration of Biotin 5γ/l, Desthiobiotin 5γ/l, and 7, 8-Diaminopelargonic acid 10γ/l, respectively.
2) In the experiment using the Glucose-Acetate mixed media devided into four parts, considerable amounts of cell growth and L-GA production were observed in the mixed medium containing 2% Glucose-Acetate.
3) In the cases of using the media containing methanol, ethanol, ethylacetate, acetic acid (free acetate), Na-acetate:NH₄-acetate=2 : 1, the production of L-GA were in decreasing order as follows; Na-Acetate:NH-Acetate=2 : 1> Acetic acid (free acetate)> Ethylacetate> Ethanol> Methanol.
4) When biotin vitamers as growth factors were added in the medium containing Glucose Acetate as the source of carbon, the substitution effect of Desthiobiotin was almost the same, 7,8-Diaminopelargonic acid 3 or 4 times stronger, and Bisnorbiotin has no substitution effect, compared with Biotin.
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